This blog will focus on three topics: rabbit antibodies, LIMK2, and custom antibody production. You can get the right solutions with superior customer service if you know where to look when shopping for custom antibody production.
Did you know a rabbit spleen contains more B cells
than that of a mouse? Getting more B
cells into the mix is important because they make antibodies, giving a better
chance of finding the right clone for expansion. In other words, that’s a good thing.
People often ask about antibodies with a wider range
of epitopes, and rightfully so as they are quite important. Immunoglobulin genes are found in rabbit antibodies which, in fact, fit an “extensive range of
epitopes.” Since the forms of a protein
differ by just one amino acid residue, it results in a slight variation in
structures. A rabbit’s immune response can develop antibodies that
are better able to detect these subtle differences. On average, rabbit
antibodies have a 10-100x higher affinity than mouse antibodies.
Two
points on the matter: Reduced Immunodominance: When
certain epitomes from the same antigen are more immunogenic than others, it is
called immunodominance. This
results in the immune system developing antibodies against the dominantly
immunogenic epitope (but few antibodies against the other epitopes). Rabbits
have less immunodominance than do mice.
More
Small Molecules are Immunogenic in Rabbits: Another benefit
ofchoosing a rabbit antibody is that the rabbit’s immune system is able to
mount an immunogenic response to small molecules that are not very
immunogenic to mice.
Rabbit antibodies might be the
right way to go for your project. The
companies that produce them also produce products related to LIMK2,
so they could be a real one-stop-shop for your research. These are the offered services: polyclonal
antibody development services, antigen preparation, vector construction to
antibody expression, and purification. The main objective is to save the client
time. Please visit www.capralogics.com
for more details.
