When utilizing mammalian or human protein antigens, the chicken is a suitable non-mammalian host animal. Chickens don’t develop high avidity antibodies to mammalian antigens, particularly those that are evolutionarily conserved because they’re not mammals.
Additionally, due to the radical changes, chicken
IgY will bind to more epitopes on a mammalian antigen (resulting in signal
development).
Additional Benefits of IgY or chicken antibodies include the following:
· Because the antibodies are contained within the yolk of the egg, the collection technique is significantly less invasive than it is for other host species.
· In a month, a single chicken can produce approximately 2-3g of antibodies.
· Proteins A/G do not link to IgY.
· Mammalian Fc receptors are not recognized by IgY antibodies.
· On average, a particular antibody response can be seen considerably faster in avian hosts than in mammalian hosts.
· Eggs can be kept at 4 degrees Celsius for at least six months without quality loss.
Goat Anti-Llama IgG (H+L) Secondary Antibody [FITC]
When somebody needs antiserum in large quantities, the
goat is a great choice for a host animal.
It suits antibody generation. Some items that require large amounts of
antibodies include host cell protein (HCP) assays, potency assays, and
immunodiagnostic tools.
The goat anti-llama FITC secondary
antibody responses only to llama IgG, heavy chains, light chains and other
llama immunoglobulins through immunoelectrophoresis and ELISA. Other elements
like antibody to serum proteins, other than immunoglobulins were not noted.
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